Protein Purification Reagents and Kits
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Filtered Search Results
Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose
Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).
| Stationary Phase | Divalent Nickel, Metal Chelate |
|---|---|
| Type | Agarose |
| Target | His-Tagged Protein |
| Content And Storage | Store at 4°C |
| Format | Bottle |
| Form | Liquid Suspension |
| Column Type | Affinity, Agarose Resin |
| Capacity (Metric) | 20 mg His-Tagged GFP Binding per mL Resin |
| Product Line | HisPur |
Thermo Scientific™ HisPur™ Cobalt Resin
Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.
| Stationary Phase | Metal Chelate, Divalent Cobalt |
|---|---|
| Type | Resin |
| Target | His-Tagged Protein |
| Content And Storage | Store at 4°C |
| Format | Bottle |
| Protein Form | Recombinant |
| Form | Liquid Suspension |
| Column Type | Affinity, Agarose Resin |
| Product Line | HisPur |
Thermo Scientific™ Pierce™ Biotinylated Protein Interaction Pull-Down Kit
Easily capture and affinity-purify binding partners of any biotin-labeled protein or other ligand using these streptavidin agarose beads and complete reagent set.
Thermo Scientific™ Pierce™ Direct Magnetic IP/Co-IP Kit
Conjugate any antibody or protein to magnetic beads and immunoprecipitate (affinity purify) target protein complexes without IP-antibody interference.
Thermo Scientific™ Tetraethylene Glycol Monooctyl Ether (C8E4), LC/MS grade
Tetraethylene Glycol Monooctyl Ether (C8E4) is a non-ionic surfactant that possesses unique properties making it suitable for solubilization of sensitive biomolecules without significant denaturation or disruption of their structure and function.
Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide
Tricosa-peptide for competitively eluting DYKDDDK-tagged fusion proteins from immobilized supports
| Shipping Condition | Wet Ice |
|---|---|
| Format | Lyophilized Powder |
| Form | Lyophilized Powder |
| Protein Form | Recombinant |
| Product Type | Peptides |
| dimMatrix | Affinity Resin |
| For Use With (Application) | Protein Purification and Isolation |
| Product Line | Pierce |
Thermo Scientific™ Lauryldimethylamine-N-oxide (LDAO), LC/MS grade
Lauryldimethylamine-N-oxide (LDAO) is a zwitterionic surfactant that possesses unique properties for solubilization and stabilization of biomolecules, particularly membrane proteins.
| CAS | 1643-20-5 |
|---|---|
| Form | Powder |
| Product Type | Lauryldimethylamine-N-oxide |
| Molecular Weight (g/mol) | 229.4 |
| Color | White |
| Purity | ≥99% |
| For Use With (Application) | LC-MS |
| Molecular Formula | C14H31NO |
Thermo Scientific™ Pierce™ Glycoprotein Isolation Kit, ConA
Purify glycoproteins from serum and cell lysates using affinity resins or kits with concanavilin A (ConA) or wheat germ agglutinin (WGA) lectins.
Thermo Scientific™ ActivX™ Azido-FP Serine Hydrolase Probe
ActivX™ probes have been designed to selectively label and enrich serine hydrolases through tagged fluorophosphonate to assess enzyme activity. ActivX Serine Hydrolase Probes covalently label serine hydrolase using fluorophosphonate conjugated with either desthiobiotin or azide.
Cytiva HisTrap™ excel Prepacked Columns
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
| Type | Pre-packed Column |
|---|---|
| Content And Storage | 4°C to 30°C |
| Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 2 to 12 |
| Material | Polypropylene |
| Support Type | 6% Highly Cross-linked Agarose |
Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
| Particle Size | 90μm |
|---|---|
| Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
| Flow Rate | 600 cm/hr. |
| Height (Metric) | 250mm |
| Content And Storage | 4° to 30°C |
| pH Range | 3 to 12 |
| Product Type | Ni Sepharose 6 FF chromatography media |
| dimMatrix | Ni-Sepharose |
| Support Type | 6% Cross-linked Agarose |
| Max. Pressure | 1 bar |
| Height (English) | 9.84 in. |
Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
| Particle Size | 90μm |
|---|---|
| Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
| Flow Rate | 150 to 600cm/hr. |
| Content And Storage | 4° to 30°C |
| pH Range | 2 to 12 |
| Product Type | Ni Sepharose excel chromato graphy media |
| dimMatrix | Ni-Sepharose |
| Support Type | 6% Highly Cross-linked Agarose |
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
| Flow Rate | <20 mL/min. |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C, 20% Ethanol |
| Diameter (English) Inner | 0.6 in. |
| Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Diameter (Metric) Inner | 16 mm |
| Material | Polypropylene |
| Support Type | 6% Cross-linked Agarose |
Thermo Scientific™ Pierce™ ChIP-Grade Protein A/G Plus Agarose
Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.
| Stationary Phase | Protein A/G |
|---|---|
| Type | Agarose |
| Target | IgG Antibody |
| Content And Storage | Upon receipt store at 4°C |
| Format | Bottle |
| Form | Liquid Suspension |
| Column Type | Affinity, Agarose Resin |
| Product Line | Pierce |
Thermo Scientific™ HisPur™ Cobalt Superflow Agarose
Perform high-purity, large-scale purification of polyhistidine-tagged proteins with this tetradentate-chelating Superflow 6 support charged with divalent cobalt.
| Stationary Phase | Metal Chelate, Divalent Cobalt |
|---|---|
| Type | Agarose |
| Target | His-Tagged Protein |
| Content And Storage | Store at 4°C |
| Format | Bottle |
| Form | Liquid Suspension |
| Column Type | Affinity, Agarose Resin |
| Product Line | HisPur |